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Paul Walther
 Elektronenmikroskopie Uni Ulm
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Ausgewählte Publikationen in reviewten Zeitschriften
selected publications in peer reviewed journals

Walther P. High-resolution cryo-SEM allows direct identification of F-actin at the inner nuclear membrane of Xenopus oocytes by virtue of its structural features. J Microsc. (in press)

Dausend J, Musyanovych A,  Dass M, Walther P, Schrezenmeier H, Landfester K, Volker Mailänder V. (2008) Uptake Mechanism of Oppositely Charged Fluorescent Nanoparticles in HeLa Cells. Macromolecular Bioscience [Epub ahead of print]

Felder E,  Siebenbrunner M, Busch T, Fois G, Miklavc G, Walther P,  Dietl P. Mechanical strain of alveolar type II cells in culture: Changes in the transcellular cytokeratin network and adaptations. Am J Physiol. [Epub ahead of print]

Walther P. (2008) High Resolution Cryoscanning Electron Microscopy of Biological Samples. Book article in: Schatten H, Pawley J. B. (eds) Biological Low-Voltage Scanning Electron Microscopy. Springer New York. ISBN 978-0-387-72970-1 (Print) 978-0-387.72972-5 (Online). pp 245-261. Summary

Schlottmann S, Buback F, Stahl B, Meierhenrich R, Walter P, Georgieff M, Senftleben U. (2008) Prolonged Classical NF-κB Activation Prevents Autophagy upon E. coli Stimulation In Vitro: A Potential Resolving Mechanism of Inflammation. Mediators of Inflamation doi:10.1155/2008/725854 (in press)

Junglas B, Briegel A, Burghardt T, Walther P, Wirth R, Huber H, Rachel R. (2008)
Ignicoccus hospitalis and Nanoarchaeum equitans: Ultrastructure, cell-cell interaction, 4 and 3D reconstruction from serial sections of freeze-substituted cells and by electron cryotomography. Arch Microbiol (in press)

Arnold M, Hirschfeld-Warneken VC, Lohmüller T, Heil P, Blümmel J, Cavalcanti-Adam EA, Lopez-Garcia M, Walther P, Kessler H, Geiger B, Spatz JP. (2008) Induction of Cell Polarization and Migration by a Gradient of Nanoscale Variations in Adhesive Ligand Spacing. Nano Lett. 8(7):2063-2069 (Epub ahead of print)

Lorenz MR, Kohnle M-V, Dass M, Walther P, Höcherl A, Ziener U,  Landfester K, Mailänder V. (2008) Synthesis of Fluorescent Polyisoprene Nanoparticles and their Uptake into Various Cells. Macromol. Biosci. 2008, 8, DOI: 10.1002/mabi.200700336

Gessert S, Marus D, Brade D, Walther P, Pandur P, Kuehl M. DM-GRASP/ALCAM/CD166 is required for cardiac morphogenesis and maintenance of cardiac identity in first heart field derived cells. Developmental Biology 2008 Jun 16. [Epub ahead of print]).

Barbosa-Barros L, de la Maza A, Estelrich J, Linares AM, Feliz M, Walther P, Pons R, López O. (2008) Penetration and Growth of DPPC/DHPC Bicelles Inside the Stratum Corneum of the Skin. Langmuir. 24(11):5700-62008  Summary

von Wichert G, Edenfeld T, von Blume J, Krisp H, Krndija D, Schmid H, Oswald F, Lother U, Walther P, Adler G, Seufferlein T. (2008) Protein kinase D2 regulates chromogranin A secretion in human BON neuroendocrine tumour cells. Cell Signal. 2008 May;20(5):925-34. Epub 2008 Jan 16. Summary

Hölzle A, Fischer S, Heyer R, Schütz S, Zacharias M, Walther P, Allers T, Marchfelder A. (2008). Maturation of the 5S rRNA 5' end is catalyzed in vitro by the endonuclease tRNase Z in the archaeon H. volcanii. RNA. 2008 Mar 27; [Epub ahead of print] Summary

Mailänder V, Lorenz MR, Holzapfel V, Musyanovych A, Fuchs K, Wiesneth M, Walther P, Landfester K, Schrezenmeier H. (2008). Carboxylated Superparamagnetic Iron Oxide Particles Label Cells Intracellularly Without Transfection Agents. Mol Imaging Biol. 2008 Feb 23; [Epub ahead of print] Summary

Musyanovych A, Schmitz-Wienke J, Mailänder V, Walther P,  Landfester K. (2008). Preparation of Biodegradable Polymer Nanoparticles by Miniemulsion Technique and Their Cell Interactions. Macromolecular Bioscience, 11, 127-136. Summary

Dass M,  Höcherl A, Kohnle M-V,  Landfester K,  Lorenz M, Mailänder V,  Walther P,  Ziener U. Carboxylated Superparamagnetic Iron Oxide Particles Label Cells Intracellularly Without Transfection Agents. Molecular Imaging & Biology, accepted

Barbosa-Barros L, de la Maza A, Walther P, Estelrich J and  López O. (2008) Morphological effects of ceramide in DMPC/DHPC bicelles J Microsc. 230, 16-26. Summary

Buser C, Walther P. (2008) Freeze-Substitution: The Addition of Water to Polar Solvents Enhances the Retention of Structure and Acts at Temperatures Around –60 °C. J. Microsc. 230, 268-77. Summary

Martin R, Hild S, Walther P, Ploss K, Boland W, Tomaschko K.-H. (2007) Granular Chitin in the Epidermis of Nudibranch Molluscs. Biol. Bull. 213, 307-315. Summary

Tournaviti S, Hannemann S, Terjung S, Kitzing TM, Stegmayer C, Ritzerfeld J, Walther P, Grosse R, Nickel W, and Fackler OT. SH4 Domain-Induced Plasma Membrane Dynamization Promotes Bleb-Associated Cell Motility. Journal of Cell Science. in press

Rupp B, Ruzsics Z, Buser C, Adler B, Walther P, Koszinowski UH. (2007) A random screen for dominant-negative mutants of the cytomegalovirus nuclear egress protein M50. J Virol. [Epub ahead of print]

Buser C, Walther P, Mertens T, Michel D. (2007) Cytomegalovirus primary envelopment occurs at large infoldings of the inner nuclear membrane.
J Virol. 81, 3042-8. Summary


Buser C, Fleischer F, Mertens T, Michel D, Schmidt V, Walther P. (2007) Quantitative investigation of murine cytomegalovirus nucleocapsid interaction. J. Mircrosc. 228, 78-87. Summary

Martin R, Tomaschko K-H, Walther P. (2007) Protective skin structures in shell-less marine gastropodes. Marine Biology, Vol. 150: 807-817.

J. Eisenmenger J, Oettinger M, Pfahler C, Plettl A, Walther P, Ziemann P. (2007) Temperature-dependent matching in a flux-line lattice interacting with a triangular array of pinning centers without long-range order. PHYSICAL REVIEW B 75, 144514.

Gorb SN, Niederegger S, Hayashi CY, Summers AP, Vötsch W, Walther P (2006). Silk-like secretion from tarantula feet. nature 443, 407. Summary

Reinhardt B, Winkler M, Schaarschmidt P, Pretsch R, Zhou S, Vaida B, Schmid-Kotsas A, Michel D, Walther P, Bachem M and Mertens T.  (2006) Human cytomegalovirus-induced reduction of extracellular matrix proteins in vascular smooth muscle cell cultures: a pathomechanism in vasculopathies? J Gen Virol. 87, 2849-2858. Summary

Lorenz MR,  Holzapfel V,  Musyanovych A,  Nothelfer K, Walther P, Frank H,  Landfester K,  Schrezenmeier H, Mailänder V. (2006) Uptake of Functionalized, Fluorescent Labeled Polymeric Particles in Different Cell Lines and Stem Cells. Biomaterials, 27, 2820-2828. Abstract

Beil M, Eckel S, Fleischer F, Schmidt H, Schmidt V, Walther P. (2006) Fitting of random tesselation models to keratin filament networks. J of Theoretical Biology, 241, 62-72. Abstract

Weigl F, Fricker S, Boyen H-G, Dietrich Ch, Koslowski B, Plettl A, Pursche O, Ziemann P, Walther P, Hartmann Ch, Ott M, Möller M (2006). From self-organized masks to nanotips: A new concept for the preparation of densely packed arrays of diamond field emitters. Diamond and Related Materials, 15, 1689-1694.

Beil M, Braxmeier H, Fleischer F, Schmidt V, Walther P (2005) Quantitative analysis of keratin  filament networks in scanning electron microscopy images of cancer cells. J Microsc. 220, 84-95. Abstract

Fabritius H, Walther P, Ziegler A (2005) Architecture of the organic matrix in the sternal CaCO3 deposits of Porcellio scaber (Crustacea, Isopoda) J Struct Biol 150:190-199. Abstract

Wild P, Ziegler U, Schraner EM, Senn C, Tobler K, Engels M, Loepfe E, Ackermann M, Mueller M, Walther P. (2005)  Impairment of Nuclear Pores in Bovine Herpesvirus 1 Infected MDBK Cells. J. Virol. 79, 1071-1083. Abstract

Rustom A, Saffrich R, Markovic I, Walther P, Gerdes H-H. (2004) Nanotubular Highways for Intercellular Organelle transport. SCIENCE, 303, 1007-1010. Abstract

Nijsse J, Walther P and  Hoekstra FA. (2004) Cold-induced imbibition damage of lettuce embryos: A study using cryo-scanning electron microscopy. Seed Science Research, 14, 117-126.  Abstract

Kjeken R, Egeberg M, Habermann A, Kuehnel M, Peyron P, Floetenmeyer M, Walther P, Jahraus A, Defacque H, Kuznetsov S and Griffiths G. (2004) Fusion between phagosomes, early and late endosomes; a role for actin in late, but not early fusion events. Mol Biol Cell, 15, 345-358 Abstract

Lopez O, Lopez-Iglesias C, Cocera M, Walther P, Parra JL, De La Maza A.(2004) Influence of chemical and freezing fixation methods in the freeze-fracture of stratum corneum. J Struct Biol.  146: 302-309. Abstract

Beil M , Micoulet A, von Wichert G, Paschke S, Walther P, Omary MB, Van  Veldhoven PP, Gern U, Wolff-Hieber E, Eggermann J, Waltenberger J, Adler G, Spatz J and Seufferlein  T (2003) Sphingosylphosphorylcholine regulates keratin network architecture and visco-elastic properties of human cancer cells. Nature Cell Biology, 5, 803-811. (With cover page) Abstract

Schneider EM, Lorenz L, Walther P and Janka-Schaub GE (2003) Natural Killer Deficiency: A Minor or Major Factor in the Manifestation of Hemophagocytic Lymphohistiocytosis? Journal of Pediatric Hematology/Oncology, 25, 680-683.

Lengl G, Ziemann P, Banhart F, Walther P. (2003) Anomalous behavior of gold nanoislands on top of SrTiO3 (001) during their overgrowth by thin YBaCuO films. Physica C 390, 175-184.

Walther P (2003) Recent progress in freeze fracturing of high-pressure frozen samples. J Microsc, 212, 34-43.

Walther, P. (2003) Cryo-fracturing and cryo-planning for in-lens cryo-SEM, using a newly designed diamond knife. Microscopy and Microanalysis 9, 279-285.

Walther P (2003) The potential of high resolution cryo-SEM in life science. Hitachi Instrument News 40, 3-8. (not reviewed)

Martin R, Walther P (2003) Protective mechanisms against the action of nematocysts in the epidermis of cratena peregrina and flabellina affinis (Gastropoda, Nudibranchia). Zoomorphology 122, 25-35

Walther P, Ziegler A.(2002) Freeze Substitution of High-Pressure Frozen Samples: The Visibility of Biological Membranes is Improved when the Substitution Medium Contains Water. Journal of Microscopy 208, 3-10. 

Sarnatskaya VV, Lindup WE, Walther P, Maslenny VN, Yushko LA, Sidorenko AS, Nikolaev AV, Nikolaev VG. (2002) Albumin, bilirubin, and activated carbon: new edges of an old triangle. Artif Cells Blood Substit Immobil Biotechnol 2002 Mar;30(2):113-26 

Martin R, Walther P (2002) Effects of discharging nematocysts when an eolid nudibranch feeds on a hydroid. J Mar Biol Ass UK 82, 455-462. 

Wild P, Schraner E M, Cantieni D, Loepfe E, Walther P, Müller M, Engels M. (2002) The significance of Golgi-Complex in Envelopment of Bovine Herpesvirus 1 (BHV-1) as Revealed by Cryobased Electron Microscopy. Micron 33, 327-337. 

Schlatterer C, Walther P, Müller M, Mendgen K, Zierold K, Knoll G. (2001) Calcium stores in differentiated Dictyostelium discoideum: prespore cells sequester calcium more efficiently than prestalk cells. Cell Calcium 29, 171-182. 

Lopez O, Cocera M, Walther P, Wehrli E, Coderch L, Parra JL, de la Maza A. (2001) Liposomes as protective agents of stratum corneum against octyl glucoside: a study based on high-resolution, low-temperature scanning electron microscopy. Micron. 32, 201-205. 

Epperlein HH, Radomski N, Wonka F, Walther P, Wilsch M, Muller M, Schwarz H. (2000)  Immunohistochemical Demonstration of Hyaluronan and Its Possible Involvement in Axolotl  Neural Crest Cell Migration. J Struct Biol. 132, 19-32 

Lopez O, Walther P, Cocera M, de La Maza A, Coderch L, Parra JL (2000) Structural Modifications in the Stratum corneum by Effect of Different Solubilizing Agents: A Study Based on High-Resolution Low-Temperature Scanning Electron Microscopy. Skin Pharmacol. Appl Skin Physiol. 13, 265-272 

Beginn U, Zipp G, Mourran A, Walther P and Möller M. (2000) Membranes Containing Oriented Supramolecular Transport Channels. Adv. Mater. 12 (7) 513-516 

Walther P and Müller M.(1999) Biological ultrastructure as revealed by high resolution cryo-SEM of blockfaces after cryo-sectioning. J. Microsc, 196 (3) 279-287 

Kääb MJ, Richards RG, Walther P, ap Gwynn I and Nötzli HP (1999)
A COMPARISON OF FOUR PREPARATION METHODS FOR THE MORPHOLOGICAL STUDY OF ARTICULAR CARTILAGE FOR SCANNING ELECTRON MICROSCOPY. Scanning Microscopy Vol. 13, No. 1, 1999 (Pages 61-69) 0891-703599$5.00+.25
Scanning Microscopy International, Chicago (AMF O’Hare), IL 60666 USA

Weisbach C, Walther P, Hartwig UA, Nösberger J. (1999) Electron Microscopical Investigation of Water Occlusions in Intercellular Spaces in the Inner Cortex of Lucerne Nodules. J. Struct. Biol. 126. 59-71. 

Spieser F, Graf B, Walther P, Noesberger J. (1998) Impact of Apple Rust Mite (Acari: Eriophyiidae) Feeding on Apple Leaf Gas Exchange and Leaf Color Associated with Changes in Leaf Tissue. Environ Entomol 27 (5), 1149-1165. 

Eppenberger-Eberhardt M, Aigner S, Donath MY, Kurer V, Walther P, Zuppinger C, Schaub MC, Eppenberger HM (1997) IGF-I and bFGF differentially influence atrial natriuretic factor and alpha-smooth muscle actin expression in cultured atrial compared to ventricular adult rat cardiomyocytes. J Mol Cell Cardiol  29(8), 2027-2039 

Frey B, Brunner I, Walther P, Scheidegger C, Zierold K. (1997) Element localization in ultrathin cryosections of high pressure frozen ectomycorrhizal sprouce roots. Plant Cell and Environment 20, 929-937. 

Kyprianidou-Leodidou T, Margraf P, Caseri W, Suter U W, Walther P. (1997) Polymer sheets with a thin nanocomposite layer acting as a UV filter. Polymers for advanced technologies 8, 505-512. 

Dreher F, Walde P, Walther P, Wehrli E. (1997) Interaction of a lecithin microemulsion gel with human stratum corneum  and its effect on transdermal transport. J. of Controlled Release 45, 131-140. 

Walther P, Müller M. (1997) Double layer coating for field emission cryo SEM - present state and applications. Scanning 19, 343-348 

Hermann R, Walther P, Müller M. (1996) Immunogold labeling in scanning electron microscopy. Histochem Cell Biol 106, 31-39 

Szczesny PJ, Walther P, Müller M. (1996) Light damage in rod outer segments: The effects of fixation on ultrastructural alterations. Current Eye Res.15, 807-814. 

Walther P, Wehrli E, Hermann R, Müller M. (1995) Double layer coating for high resolution low temperature SEM. J Microsc. 179, 229-237. 

Kaneko Y, Walther P. (1995) Comparison of Germinating Pea Leaves Prepared by High-Pressure Freezing - Freeze Substitution and Conventional Chemical Fixation. J Electron Microsc. 44, 104-109. 

Cunin C, Handschin S, Walther P, Escher F. (1995) Structural Changes of Starch during Cooking of During Wheat Pasta. Lebensm.-Wiss. u.-Technol., 28,323-328. 

Petzinger E, Föllmann W, Blumrich M, Walther P, Hentschel J, Bette P, Maurice M, Feldmann G. (1994) Immortalization of rat hepatocytes by fusion with hepatoma cells. I. Cloning of a hepatocytoma cell line with bile canaliculi. Europ J Cell Biol. 64, 328-338. 

Walther P, Chen Y, Malecki M, Steffen Zoran SL, Schatten GP, Pawley JB. (1993). Scanning Electron Microscopy of High Pressure Frozen Sea Urchin Embryos. Scanning Microscopy, Vol. 7, 4, 1283-1293. 

Zimmermann L, Weibel M, Caseri W, Suter UW, Walther P. (1993). Polymer Nanocomposites with "Ultralow" Refractive Index. Polymers for Advanced Technologies. 4: 1-7. 

Hentschel H, Walther P. (1993). Heterogeneous Distribution of glycoconjugates in the kidney of dogfish Scyliorhinus caniculus (L.) With reference to changes in the glycosilation pattern during ontogenetic development of the nephron. The Anatomical Record. 235: 21-32. 

Walther P, Chen Y, Pech LL, Pawley JB. (1992) High resolution scanning electron microscopy of frozen-hydrated cells. J. Microsc. 168: 169-180. 

Walther P, Autrata R, Chen Y, Pawley JB. (1991). Backscattered electron imaging for high resolution surface SEM with a new type YAG-detector. Scanning Microscopy. 5, 301-310. 

Pawley JB, Walther P, Shih S-J, Malecki M. (1991). Early results using high resolution, low voltage, low temperature SEM. J Microsc. 161: 1-7. 

Herter P, Tresp G, Hentschel H, Zierold K, Walther P. (1991). High resolution scanning electron microscopy of inner surfaces and fracture faces of kidney tissue using cryo-preparation methods. J Microsc. 161: 375-385. 

Mueller T, Walther P, Scheidegger Ch, Reichelt R, Mueller S, Guggenheim R. (1991). Cryo preparation and planar magnetron sputtering for low temperature scanning electron microscopy. Scanning Microsc. 4, 863-876. 

Walther P, Hentschel J, Herter P, Mueller T, Zierold K. (1990). Imaging of intramembranous particles in frozen hydrated cells (Saccharomyces cerevisiae) by high resolution cryo SEM. Scanning 12: 300-307. 

Walther P, Hentschel J. (1989). Improved representation of cell surface structures by freeze substitution and backscattered electron imaging. Scanning Microsc 1989, 3, Supplement 3: 201-211. 

Walther P, Schweingruber AM, Mueller M Schweingruber ME. (1988). Morphological organization of glycoprotein containing cell surface structures in yeast. J Ultrastruct and Molecular Struct Res. 101: 123-136. 

Borneleit P, Hermsdorf T, Claus R, Walther P, Kleber H P. (1988) Effect of hexadecene-induced vesiculation on the outer membrane of acinetobacter calcoaceticus. J Gen Microbiol. 134, 1983-1992. 

Luethe N, Plattner H, Haacke B, Walther P, Mueller M. (1986). Lectin binding sites in Paramecium tetraurelia cells. I. Labelling analysis predominantly of secretory components. Histochemistry 85: 364-376. 

Autrata R, Walther P, Kriz S, Mueller M. (1986). A BSE scintillation detector in the (S)TEM. Scanning 8: 3-8. 

Walther P, Mueller M. (1985). Detection of small (5-15 nm) gold labelled surface antigens using backscattered electrons. In: Mueller M, Becker RP, Boyde A, Wolosewick JJ. (eds) Science of Biological Specimen Preparation. SEM Inc., AMF O'Hare (Chicago), Il 60666-0507, USA.195-201. 

Walther P, Mueller M, Schweingruber ME. (1984). The ultrastructure of the cell surface and plasma membrane of exponential and stationary phase cells of Schizzosaccharomyces pombe, grown in different media. Arch Microbiol 137: 128-134. 

Kaeppeli O, Walther P, Mueller M, Fiechter A. (1984). Structure of the cell surface of the yeast Candida tropicalis and its relation to hydrocarbon transport. Arch Microbiol 138: 279-282. 

Walther P, Kriz S, Mueller M, Ariano BH, Brodbeck U, Ott P, Schweingruber ME. (1984). Detection of protein A gold (15 nm) marked surface anitgens by backscattered electrons. Scan Electron Microsc 1984, II, 1251-1266. 

Walther P, Ariano BH, Kriz S, Mueller M. (1983). High resolution SEM detection of protein A gold (15 nm) marked surface antigens using backscattered electrons. Beitr Elektronenmikrosk Direktabb Oberf 16: 539-545. (not reviewed) 

Harsch M, Walther P, Weder HG, Hengartner H. (1981). Targeting of monoclonal antibody-coated liposomes in red blood cells. Biochem Biophys Res Com 103,3: 1069-1076. 

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