Software

NPS-Software

We have recently developed a new method, termed Nano-Positioning System (NPS) which allows the localisation of flexible domains in macromolecules. The method is based on single molecule FRET measurements and a structural model for the core of the macromolecule. The method is published and explained in Nature Methods.
A. Muschielok, J. Andrecka, A. Jawhari, F. Brückner,  P. Cramer and J. Michaelis, Nature Methods (2008).

Details about the Bayesian Analysis used in NPS are described in the online supplement to the Nature Methods paper.
Supporting Information

You can download here the compiled software, as well as a pdf document containing a user guide which gives a step by step description on how to use the NPS software. The software requires a Matlab runtime to be installed on the computer. If you do not have Matlab, please write an email to michaelis(at)lmu.de and we will send you a software that can be run as a standalone software.

The entire software is based on graphical user interfaces and should therefore be easy to use and comprehend. If you have any questions or comments about the software or would like to see the source code just send an email. If you use the software for your own data analysis please cite the Nature Methods paper.

• Download the Software
• Download the Documentation

A newer software with advanced capabilities (as outlined in A. Muschielok, J. Michaelis, Application of the Nano-Positioning System to the Analysis of Fluorescence Resonance Energy Transfer Networks, 2011) is available upon request. For a short comparison of the two software versions please see the following PDF: software comparison

Note: Due to Mathworks licensing policies we can only provide the necessary Matlab runtime upon request. If you do not have access to Matlab runtime please contact: Michaelis