Dr. A. Rück - Core Facility for Confocal and Multiphoton Microscopy, Ulm University
The core unit “Confocal and Multiphoton Microscopy” exists in its current form since August 2013. It functions as a life cell imaging competence center, mainly for the purposes of biomedical research. We are involved in the development and adaption of new imaging techniques, for example phosphorescence lifetime imaging, that can be helpful for other users. Scientific topics include cell-cell adhesion, protein interaction, transport processes, cell metabolism, nanoparticles, stem cell research, wound-healing, sepsis and developmental biology.
The heart of the unit consists of two laser scanning microscopes, one being an inverted LSM710 and the other an upright microscope LSM 7 MP. Both microscopes are coupled to an fs-pulsed laser to provide multiphoton microscopy. Besides spectral detection and time-resolved detection, detailed fluorescence lifetime imaging (FLIM) is now possible with the newest generation of hybrid detectors and TCSPC electronics. In addition to the LSM 710, the LSM 510 META is equipped with a spectrometer for spectral imaging which is utilized at the facility. There are also labs available for cell culturing and molecular biology.