The Genomics Core Facility of the Faculty of Medicine offers services for all working groups of the hospital, university and external companies/universities for their research. The Genomics Core Facility consists of the former chip facility and the newly established 'Next Generation Sequencing' unit.
The sequencing service includes the entire experimental planning as well as the bioinformatic evaluation and supervision of the projects (Prof. Dr. H. A. Kestler (Institute of Medical Systems Biology) & Dr. K. Holzmann (Genomics Core Facility)).
Next Generation Sequencing
In the area of Next Generation Sequencing, the Genomics Core Facility has used a HiSeq 2000 from Illumina from 2013 until the beginning of 2019. Since February 2019, the Core Facility has now a NextSeq 550, which has significantly improved flexibility with regard to the size of sequencing projects.
Sequencing on the NextSeq 550 is both cheaper and much faster than on the HiSeq. While sequencing 200 bases on the HiSeq took 10 days, sequencing 300 bases on the NextSeq takes only 29 hours.
- Whole Genome Sequencing
- Exome Sequencing
- Panel sequencing
- RNA sequencing (also from minimal amounts of RNA)
- miRNA sequencing (also from plasma, from exosomes)
- ChIP sequencing
- Microbiome sequencing
- Single Cell RNA Seq (Chromium iX)
Affymetrix GeneChip Microarrays
In the area of Microarrays, a system from Affymetrix is available as well as a microarray spotter to produce custom arrays.
- Expression profile analyses
- miRNA analyses
- SNP/Copy Number analyses
- ChIP on Chip analyses
- Sequencing of mitochondrial DNA
- Support in the establishment of custom arrays