Core Unit Extracellular Flux Analyzer

Der Seahorse Extracellular Flux Analyzer der Firma Agilent Biotechnologies ermöglicht Analysen in 96-well-Platten.

The Core Facility Extracellular Flux Analyzer operates a state-of-the-art Seahorse XFe96 Extracellular Flux Analyzer (Agilent Biotechnologies), which allows parallel analysis of mitochondrial respiration and glycolysis in real time. Analyses are performed in 96-well plates and determine oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), together providing information on oxidative phosphorylation, glycolysis and substrate utilization.

Adherent cells as well as permeabilized cells or isolated mitochondria are suitable for measurement. Depending on the scientific question, various substrates or inhibitors can be added during the measurement; ready-to-use kits are available for this purpose.

The technique is non-invasive. After the measurement the cells can be used for additional analyses.

Further information on the technology can also be found on the homepage of the manufacturer (Agilent).

Der Seahorse  Extracellular Flux Analyzer der Firma Agilent Biotechnologies ermöglicht die parallele Analyse der mitochondrialen Atmung und der Glykolyse in Echtzeit.

More Information

  • Services

    • Introductory training
    • Support in the conception, execution, and evaluation of experiments
    • Support in the optimization of experiments
    • Data analysis
    • Provision of analysis kits and reagents
    • Full service analysis
  • Devices and facilities

    • Seahorse XFe96 Flux Analyzer
    • Access to cell culture facilities
  • Research Topics

    • Cellular energy metabolism and mitochondria research
    • Obesity, diabetes and metabolic disorders
    • Ageing
    • Trauma
    • Cancer
    • Immunology
    • Stem cell research
    • Pharmacology

     

Documents

Operating rules*

User Fees *, **

* In German, please inquire if you need assistance
** Internal users only, external users please inquire.

Usage Time

Mon-Fri: 9 am to 5 pm
(after arrangement by phone)

Booking schedule

Address

Ulm University Medical Center
Department of Pediatrics and Adolescent Medicine
Michelsberg, House 16, Room E15
Eythstr. 24
89075 Ulm