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2020
iGEM_uulm
HRP
Quiz
DE
iGEM Explained Quiz
Enzyme Driven Glow Sticks (HRP)
The cofactor haem in the active centre of the horseradish peroxidase contains:
Co2+ (Cobalt)
Fe3+ (Iron)
Zn2+ (Zinc)
Mn3+ (Manganese)
Ni3+ (Nickel)
Peroxidases are enzymes which…
can break down water into hydrogen and oxygen.
give plants their green colour.
glow in the dark to scare away vampires.
can protect cells by breaking down peroxides.
give horseradish its mild flavour.
HRP is often used popular among scientists because…
it still functions trouble-free under strong basic conditions up to pH 14.
it can be coupled to other microscopic molecules and make their reactions visible and measurable through macroscopic colour changes and luminescence.
it can intercalate between DNA double strands and cause a colour change.
it was nominated for the enzyme of the year 2020.
it can be made cheaply through expression in E. coli.
Which Statement is incorrect?
Ultrapure HRP can simply be made from horseradish by using a blender.
The large and tasty selection at the weekly market in Ulm is one of many reasons to visit the city.
chemical experiments shouldn’t be performed outside a dedicated lab.
Horseradish has a strong smell and spicy taste.
The central metal ion the haem of the HRP changes between three and four positive charges.
In criminology a similar reaction mixture is used to find tiny traces of blood.
This is possible due to the addition of potassium FERROcyanide(III) which breaks down hydrogen peroxide similarly to HRP.
This is possible due to HAEMoglobin (the red blood pigment) that catalyses the degradation of hydrogen peroxide similarly to HRP.
This is possible due to the chemiluminescence of leukocytes (WHITE cells).
This is due to the chromophoric nature of thrombocytes (platelets), which enhances the glow.
This is possible due to the addition of potassium FERROcyanide(II) which breaks down hydrogen peroxide similarly to HRP.