Single-molecule Fluorescence
Idea: Distance measurement on the molecular length scale
using single-molecule FRET (smFRET)
Biophysics Lab Course, Institute of Biophysics, Ulm University
Förster Resonance Energy Transfer is investigated in single molecule experiments.
Complementary DNA oligonucleotides, where one strand carries a donor dye, while the other carries an acceptor dye, are annealed and immobilized using microfluidics on a quartz surface.
With a Total Internal Reflection Fluorescence Microscope (TIRFM), the FRET-efficiency of single-molecule FRET events is measured and used to determine the donor-acceptor distance.
Biophysics Lab Course: Gene expression | Bioinformatics | Protein labelling | Protein crystallization | Protein folding | Stopped-flow kinetics | Single-molecule fluorescence | Fluorescence lifetime | Ligand binding | Fluorescence correlation spectroscopy (FCS) | Lipid monolayers | Ion channels | Live cell imaging | Superresolution microscopy | Optical tweezers | AFM
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