Bioinformatics
Idea: In this experiment we identify and characterize proteins from short peptide sequences
using computational databases
Biophysics Lab Course, Institute of Biophysics, Ulm University
Online algorithms like BLAST, FASTA etc. are utilized to identify a protein from two short peptide fragments.
The proteomics tools of the ExPASy portal are used to explore the biochemical characteristics of the target protein.
The 3D structure of the protein is downloaded from the PDB and viewed with molecular visualization programmes such as Chimera.
ClustalOmega and Consurf tools are used to analyse homology information and find evolutionary relations for the protein of interest.
Biophysics Lab Course: Gene expression | Bioinformatics | Protein labelling | Protein crystallization | Protein folding | Stopped-flow kinetics | Single-molecule fluorescence | Fluorescence lifetime | Ligand binding | Fluorescence correlation spectroscopy (FCS) | Lipid monolayers | Ion channels | Live cell imaging | Superresolution microscopy | Optical tweezers | AFM
