Protein Crystallization
Idea: An introduction to protein crystallization using a vapor diffusion technique
Biophysics Lab Course, Institute of Biophysics, Ulm University
The protein lysozyme is crystallized with a vapour diffusion technique, here the hanging drop method.
Buffers with different pH and precipitant concentrations are used on a 24 well plate to find optimal crystallization conditions.
The success for the different solvent conditions is characterized by
size and number of grown crystals in the respective wells.
Biophysics Lab Course: Gene expression | Bioinformatics | Protein labelling | Protein crystallization | Protein folding | Stopped-flow kinetics | Single-molecule fluorescence | Fluorescence lifetime | Ligand binding | Fluorescence correlation spectroscopy (FCS) | Lipid monolayers | Ion channels | Live cell imaging | Superresolution microscopy | Optical tweezers | AFM
