Fluorescence Labelling of Proteins
Idea: Specific fluorescence labelling of the plasmaprotein CRP
and characterization by absorption and fluorescence spectroscopy
Biophysics Lab Course, Institute of Biophysics, Ulm University
The plasma protein CRP (C-reactive protein) is labelled with an amino-reactive fluorescent dye.
After separation from excess free dye using gel filtration chromatography, the degree of labelling is characterized by absorption spectroscopy.
A fluorometer is used to record the fluorescence excitation and emission spectra of the product.
Biophysics Lab Course: Gene expression | Bioinformatics | Protein labelling | Protein crystallization | Protein folding | Stopped-flow kinetics | Single-molecule fluorescence | Fluorescence lifetime | Ligand binding | Fluorescence correlation spectroscopy (FCS) | Lipid monolayers | Ion channels | Live cell imaging | Superresolution microscopy | Optical tweezers | AFM
