Fluorescence Labelling of Proteins

Idea: Specific fluorescence labelling of the plasmaprotein CRP
and characterization by absorption and fluorescence spectroscopy

Biophysics Lab Course, Institute of Biophysics, Ulm University


The plasma protein CRP (C-reactive protein) is labelled with an amino-reactive fluorescent dye.

After separation from excess free dye using gel filtration chromatography, the degree of labelling is characterized by absorption spectroscopy.

A fluorometer is used to record the fluorescence excitation and emission spectra of the product.