During my research in the working group of Prof. Dr. Annika Herwig, I focus on the activity and gene expression of hypothalamic brain areas in our model organism.
In nature, the Djungarian hamster (Phodopus sungorus) lives in the extreme conditions of the Siberian steppes. As soon as a shortening of day length to less than 13,5 hours announces winter, the nocturnal rodent starts an acclimatization period. The hamsters grow a winter fur for better insulation capacity. Food intake and consequently body weight are reduced. Furthermore, reproductive organs are regressed. These morphological and physiological changes serve as prerequisites for the expression of spontaneous daily torpor (SDT), which is a metabolic energy saving mechanism.
In laboratory conditions, shifting the dark-light-phase to winter-like conditions of 8 hours light and 16 hours darkness per day is sufficient to induce the short day acclimation, which takes about 12 weeks. Although our hamsters are not energetically challenged, because they have food and water ad libitum as well as a room temperature within their comfort range, they voluntarily reduce their body mass by 25 %, grow a winter fur and express spontaneous daily torpor.
To observe the course of the hamsters' body temperature, which is an indicator of the current metabolic state, a telemetry device is implanted under anaesthesia. During the experiment, the hamsters can do their usual hamster business, like eating, sleeping, building nests, and expressing torpor. The understanding of the torpor regulation shall be improved by comparing the brain of torpid and non-torpid short day acclimated “winter” hamsters at different time points with diverse methods as following:
Step 1: Identification of distinct hypothalamic regions active in torpor regulation with immunohistochemistry
Step 2: Microanatomical laser dissection of most active hypothalamic regions during torpor entrance
Step 3: Gene expression analysis of signalling mechanisms with RNA Seq (NGS)
Step 4: Anatomical verification and location of candidate genes with in situ hybridization
My PhD project is supported by the German Research Foundation (DFG).
Besides my work on my doctoral thesis I also contribute to the institute's teaching by helping in the Masters' lab courses, supervising bachelor theses, and giving lectures. To educate educates me as well.
Bachelor thesis "Simulation von Tierkämpfen", supervised by Dr. Andrea Wirmer in the working group of Prof. Dr. Harald Wolf
Master thesis "Locomotion and walking parameters of the scorpion Mesobuthus eupeus", supervised by Dr. Sarah Pfeffer in the working group of Prof. Dr. Harald Wolf
In progress: doctoral thesis “Neuroanatomical mapping of hypothalamic core areas involved in the regulation of spontaneous daily torpor in the Djungarian hamster Phodopus sungorus”, supervised by Dr. Victoria Diedrich in the working group of Prof. Dr. Annika Herwig
Conference, symposia and meeting contributions with poster presentations
Apr 2016: 9th Student Symposium on Molecular Medicine Ulm (Bachelor Thesis)
May 2017: 10th Student Symposium on Molecular Medicine Ulm (Master Thesis)
Sep 2017: 110th Annual Meeting of the German Zoological Society (DZG) Bielefeld (Master Thesis)
May 2018: 11th Student Symposium on Molecular Medicine Ulm (Doctoral Thesis)
Mar 2019: 6th Schram Foundation Symposium Göttingen
Mar 2019: 13th Göttingen Meeting of the German Neuroscience Society (NWG) Göttingen
Mar 2019: European DSI User Group meeting Berlin
May 2019: 12th Student Symposium on Molecular Medicine Ulm
Version of 13 March 2019
- Elena Haugg
- Room: M25/5207
- Phone: +49 (0) 731-50-22672
- Fax: +49 (0) 731-50-22629