Core Facility Manager
Dr. Christian Bökel
Tel: 0731 50 33700
The ULMTeC Core Facility Light Microscopy can be accessed by all scientists of the Medical Faculty and Ulm University and offers a wide spectrum of optical microscopy approaches. Our main technique, confocal microscopy, is based on the pixelwise scanning with illumination lasers to generate virtual 2D optical sections through 3D objects.
The core facility primarily provides training that allows users to later operate our microscopes independently. In addition, we offer support with the evaluation and interpretation of the image data, in particular concerning quantitative and statistical image analysis. Due to our long standing expertise we can also offer help with the design of imaging based experiments and the development of bespoke experimental protocols.
Equipment
Leica STELLARIS FALCON DIVE
- Inverse confocal microscope
- Equipped with a CW 405nm laser for DAPI excitation, a pulsed white light laser for freely selectable excitation wavelengths between 450nm and 800nm, as well as a Spectra Physics MaiTAi 2-photon laser tunable from 690nm to 1040 nm
- Five highly sensitive detectors for confocal imaging
- Two spectral non-descanning detectors for multiphoton applications (2P imaging, second harmonic generation (SHG) microscopy, metabolic FLIM)
- Optimized for multiplex microscopy and time-resolved imaging techniques
- With incubator and climate control for live cell measurements
Leica TCS SP8
- Inverse confocal microscope
- Equipped with a stage incubator for live cell imaging
HP Fury Z8 Image processing workstation
- Workstation with 12 processor cores and 128 GB RAM
- Microscopy software bundles for quantitative, offline analyses
- Usable either in the Core Facility or remotely
- Hands-on support available for image statistics or coding of e.g. Image J macros and Python scripts
Services
List of services
Facility services include:
- Standard and multiphoton confocal microscopy
- Live cell imaging
- Time resolved techniques such as FLIM, FLIM/FRET or FCCS
- Expertise in experiment design, staining protocols, subcellular imaging, …
- Support with quantitative image analysis and image statistics
- Hands on support for complex imaging tasks