The Core Facility Extracellular Flux Analyzer operates a state-of-the-art Seahorse XFe96 Extracellular Flux Analyzer (Agilent Biotechnologies), which allows parallel analysis of mitochondrial respiration and glycolysis in real time.

The services provided include:

• Introductory training
• Support in the conception, execution, and evaluation of experiments
• Support in the optimization of experiments
• Data analysis
• Provision of analysis kits and reagents
• Full service analysis

Analyses are performed in 96-well plates and determine oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), together providing information on oxidative phosphorylation, glycolysis and substrate utilization. Adherent cells as well as permeabilized cells or isolated mitochondria are suitable for measurement. Depending on the scientific question, various substrates or inhibitors can be added during the measurement; ready-to-use kits are available for this purpose. The technique is non-invasive. After the measurement the cells can be used for additional analyses.
Further information on the technology can also be found on the homepage of the manufacturer (Agilent).

Further equipment

• Access to cell culture facilities
• Heratherm Midi 40 compact incubator, IMC18 incubator