Contact
Head:
Prof. Dr. Pamela Fischer-Posovszky
Pamela.Fischer(at)uniklinik-ulm.de
Tel: +49 (0)731/500-57415
Deputy:
PD Dr. Daniel Tews (scientific support and appointments)
Daniel.Tews(at)uniklinik-ulm.de
Tel: +49 (0)731/500-57418
The Core Facility Extracellular Flux Analyzer operates a state-of-the-art Seahorse XFe96 Extracellular Flux Analyzer (Agilent Biotechnologies), which allows parallel analysis of mitochondrial respiration and glycolysis in real time. Analyses are performed in 96-well plates and determine oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), together providing information on oxidative phosphorylation, glycolysis and substrate utilization.
Adherent cells as well as permeabilized cells or isolated mitochondria are suitable for measurement. Depending on the scientific question, various substrates or inhibitors can be added during the measurement; ready-to-use kits are available for this purpose.
The technique is non-invasive. After the measurement the cells can be used for additional analyses.
Further information on the technology can also be found on the homepage of the manufacturer (Agilent).
Head:
Prof. Dr. Pamela Fischer-Posovszky
Pamela.Fischer(at)uniklinik-ulm.de
Tel: +49 (0)731/500-57415
Deputy:
PD Dr. Daniel Tews (scientific support and appointments)
Daniel.Tews(at)uniklinik-ulm.de
Tel: +49 (0)731/500-57418
Operating rules*
User Fees *, **
* In German, please inquire if you need assistance
** Internal users only, external users please inquire.
Ulm University Medical Center
Department of Pediatrics and Adolescent Medicine
Michelsberg, House 16, Room E15
Eythstr. 24
89075 Ulm