Core Unit Mass Spectrometry and Proteomics

Das Banner-Titelbild stellt die Methodik der Core Unit Massenspektrometrie und Proteomics von Proteinidentifizierung über -aufreinigung bis zur Analyse und Quantifizierung dar.

The ULMTeC Core Unit Mass Spectrometry and Proteomics (CUMP) offers all research groups of the Ulm Medical Centre and the University of Ulm as well as external companies and academic institutions the possibility of state-of-the-art proteome research for the identification and quantification of proteins and post-translational modifications. Customised mass spectrometric solutions on ESI or MALDI instruments are offered, complemented by efficient data analysis tools. From planning and experimental design to practical application tips, you will receive detailed advice from CUMP staff.

QuickLinks

Project registration Peptide libraries Booking schedules Agendo

Services

Die Orbitrap Velos Pro erlaubt die Identifizierung und Quantifizierung tausender Proteine. — Orbitrap Elite

With SDS-PAGE and SCX chromatography, potent methods are available for sample fractionation. Various fragmentation techniques (CID, HCD, MSA) can be used for mass spectrometric analyses.

A large number of different programmes and solutions are available for data evaluation and statistical analysis. The methods and technologies established at CUMP enable the processing of a broad spectrum of biological and medical issues. Both complex qualitative and quantitative analyses can be carried out using all common methods (label-free, SILAC, isobaric reagents).

Services:

Protein/peptide fractionation (SDS-PAGE, SCX chromatography)
Proteolytic digestion
Peptide enrichment (TiO2 enrichment of phosphopeptides)
Mass spectrometric analysis
Bioinformatic data analysis (qualitative and quantitative)
Statistical analysis

Equipment

Product image MALDI-TOF — Axima Confidence MALDI-TOF mass spectometer

Orbitrap Elite

The CUMP currently operates a Thermo Scientific Orbitrap Elite, which is coupled online with a Dionex RSLCnano uPLC. This combination allows the identification and quantification of thousands of proteins and post-translational modifications.

HPLC Anlage

CUMP also operates a multi-dimensional HPLC system for the separation of peptides and proteins using cation exchange chromatography (SCX) or size exclusion chromatography (SEC).

MALDI-TOF

The AXIMA-Confidence offers the required detection sensitivity with excellent mass accuracy and mass resolution for unambiguous identification of the proteins of interest.

More Information

ULMTeC operating and usage regulations^*,**(see Teil B and C)

User fees^*,**

^{* In German, please inquire if you need assistance.}
^{*,** Internal users only, external users please inquire.}

1. Please send requests for analyses to med.proteomics(at)uni-ulm.de.

2. When submitting the sample, please bring the completed application form with the necessary project details (e.g. cost account).

• Proteomics
• Identification of bioactive peptides
• PTM analysis

Here you can find more information about the available Peptide libraries.

The following posters demonstrate the current research activities performed at the CUMP.

As part of two research consortia, CUMP is performing extensive method development in fields such as intact protein analysis, PTM enrichment, and endogenous peptide identification. The following posters outline our current efforts in these fields.

In accordance with § 7 of the Operating rules all users of the Core Facility are obligated to acknowledge the facility appropriately in any type of publication. Suggested formulations and the DFG project numbers associated with the CF research infrastructure can be found here.

Core Unit Mass Spectrometry and Proteomics
Medizinische Fakultät Universität Ulm
N26, Raum 5209 (5. Stock, durch 4. Stock Ostflügel)
Albert-Einstein-Allee 11
89081 Ulm
Labor:
N26, Raum 4204 (4. Stock, Ostflügel)