Ulm University

Congratulations to Stephanie Hampp and Melanie Rall to their paper which was published online this week in PNAS

Stephanie Hampp and Melanie Rall, both members of the IGradU in the Division of Gyneacological Oncology, Department of Obstetrics and Gynaecology, could successfully publish their data under the supervision of Prof. Dr. Lisa Wiesmüller in Proc Natl Acad Sci U S A (Hampp et al. 2016 PNAS). In this project the authors could characterize a novel damage tolerance pathway that requires the combined function of DNA polymerase iota (POLɩ) and p53. They provide evidence (including the usage of the DNA fiber spreading technique) demonstrating that p53 complexed with POLɩ triggers idling events that decelerate nascent DNA elongation at replication barriers, facilitating the resolution of stalled forks by specialized structure-specific enzymes. These findings implicate p53 in the protection of quickly growing cancer cells verifying our previous study (Ireno et al. 2014 Carcinogenesis) showing that p53 shows a healer-function in breast-cancer cells treated with PARP-inhibitors and also implicate p53 in the protection of hematopoietic stem cells undergoing excessive self-renewal. One cooperation partner in this project was Dr. Vanesa Gottifredi (head of the Cell Cycle and Genomic Stability Laboratory of the Fundación Instituto Leloir, Buenos Aires, Argentina) who also was a Guest Scientist of the IGradU in November 2015. During a practical training in 2014, financed by the Mobility Programme of the IGradU, Stephanie Hampp was able to learn the DNA fiber spreading technique under the supervision of Dr. Vanesa Gottifredi which was key to the success of the project now published in PNAS.