The ULMTeC Core Facility Functional Peptidomics (CFP) originated from the competence centre "Ulm Peptide Pharmaceuticals" (U-PEP) and offers its expertise in the field of bioactive peptides to the working groups of the Medical Faculty and the University of Ulm as well as to external users.

This includes the discovery of new active peptide substances from natural sources by means of chromatography, as well as their chemical synthesis, modification and folding. The CFP has preparative and analytical chromatography systems (HPLC), multidimensional HPLC, pipetting robots, freeze-drying equipments, peptide synthesisers and chromatography-coupled mass spectrometry (LC-MS) at its disposal for this work.

In addition to providing existing peptide libraries for bioscreening programs, the Core Facility can process tissues, body fluids and cell supernatants for special project requirements with the aim of extracting, fractionating and ultimately identifying bioactive peptides from them. Identified peptide active substances can then be chemically resynthesised and their potency or stability improved by structure-activity-relationship (SAR) analysis.

QuickLinks

Peptide libraries  CRC 1279

• Peptide libraries for bioscreening - projects
• Peptide synthesis (solid phase chemical synthesis)
• Peptide derivatization and peptide folding
• Peptide extraction from tissues and fluids
• Fractionation via HPLC
• Peptide analysis
• Freeze-drying

Preparative and analytical chromatography (HPLC)

• Varian PrepStar SD-1 (purification/separation on a gram scale)
• Hitachi Chromaster 5430 (purification/separation on a microgram scale)

Chromatography-coupled mass spectrometry (LC-MS)

• Waters 2790 + Waters micromass ZQ

Microwave peptide synthesiser Liberty Blue (CEM)

• Peptide syntheses up to 50 amino acids - chain length at desired scale and purity

Liquid-Handler and 2D-HPLC

• Gilson GX 281

Freeze-drying

• Christ-Epsilon 1-6 (up to 6 L volume per drying)

• Scientific advice on identifying new peptide substances from natural sources
• Support in planning and implementing peptide-based screening strategies
• Advice on selecting suitable extraction, fractionation and analysis methods
• Strategic support in the structure function analysis of peptides for active optimization
• Support in methodical adaptation for project-specific requirements
• Development of individual synthesis and modification strategies for bioactive peptides
• Advice on creating peptide libraries