Publications 2024

Hecht M, Alber N, Marhoffer P, Johnsson N, Gronemeyer T.
The concerted action of SEPT9 and EPLIN modulates the adhesion and migration of human fibroblasts.
Liefe Sci Alliance. 2024 7(7): e202201686

Litsios A, Grys BT, Kraus OZ, ..., Timmermann S, Billmann M, Myers C, Johnsson N, Churchman LS, Boone C, Andrews BJ.
Proteome-scale movements and compartment connectivity during the eukaryotic cell cycle.
Cell. 2024 187(6):1490-1507.e21. doi: 10.1016/j.cell.2024.02.014.

Grupp B, Denkhaus L, Gerhardt S, Vögele M, Johnsson N, Gronemeyer T.
The structure of a tetrameric septin complex reveals a hydrophobic element essential for NC-interface integrity
Commun Biol. 2024 7(1):48. doi: 10.1038/s42003-023-05734-w. 

Publications 2023

Müller J, Furlan M, Settele D, Grupp B, Johnsson N.
Transient septin sumoylation steers a Fir1-Skt5 protein complex between the split septin ring.
J Cell Biol 2024 J223(1):e202301027

Grupp B, Lemkul J, Gronemeyer T.
An in silico approach to determine inter-subunit affinities in human septin complexes.
Cytoskeleton (Hoboken) 2023 80, 141-152

Publications 2022

Grupp B, Gronemeyer T.
A biochemical view on the septins, a less known component of the cytoskeleton.
Biol Chem. 2022; 404(1):1-13. doi: 10.1515/hsz-2022-0263

Fischer M, Frank D, Rösler R, Johnsson N, Gronemeyer T.
Biochemical characterization of a human septin octamer
Front. Cell Dev. Biol. 2022; 10: 771388

Publications 2021

Duan X, Chen X, Wang K, Chen L, Glomb O, Johnsson N, Feng L, Zhou XQ, Bi E.
Essential role of the endocytic site-associated protein Ecm25 in stress-induced cell elongation.
Cell Rep. 2021 May 18;35(7):109122. doi: 10.1016/j.celrep.2021.109122.

Dünkler A, Leda M, Kromer JM, Neller J, Gronemeyer T, Goryachev AB, Johnsson N.
Type V myosin focuses the polarisome and shapes the tip of yeast cells.
J Cell Biol. 2021 May 3;220(5):e202006193. doi: 10.1083/jcb.202006193.

Publications 2020

Grinhagens S, Dünkler A, Wu Y, Rieger L, Brenner P, Gronemeyer T, Mulaw MA, Johnsson N.
A time-resolved interaction analysis of Bem1 reconstructs the flow of Cdc42 during polar growth.
Life Sci Alliance. 2020 Jul 31;3(9):e202000813. doi: 10.26508/lsa.202000813.

Chollet J, Dünkler A, Bäuerle A, Vivero-Pol L, Mulaw MA, Gronemeyer T, Johnsson N.
Cdc24 interacts with the septins to create a positive feedback during bud site assembly in yeast.
J Cell Sci. 2020 Apr 23. pii: jcs.240283. doi: 10.1242/jcs.240283.

Glomb O, Wu Y, Rieger L, Rüthnick D, Mulaw MA, Johnsson N.
The cell polarity proteins Boi1 and Boi2 direct an actin nucleation complex to sites of exocytosis in Saccharomyces cerevisiae.
J Cell Sci. 2020; 133(3). pii: jcs237982. doi: 10.1242/jcs.237982.

Publications 2019

Glomb O, Bareis L, Johnsson N.
YFR016c/Aip5 is part of an actin nucleation complex in yeast.
Biol Open. 2019 Aug 9; 8(8).

Hecht M, Rösler R, Wiese S, Johnsson N, Gronemeyer T.
An interaction network of the human SEPT9 established by quantitative mass spectrometry.
G3 (Bethesda). 2019. Jun 5; 9(6):1869-1880

Publications 2018

Baur J, Rösler R, Wiese S, Johnsson N, Gronemeyer T.
Dissecting the nucleotide binding properties of the Septins from S. cerevisiae.
Cytoskeleton (Hoboken). 2018 Aug 9. (epub ahead of print). doi: 10.1002/cm.21484.

Publications 2017

Kustermann J, Wu Y, Rieger L, Dedden D, Phan T, Walther P, Dünkler A, Johnsson N.
The cell polarity proteins Boi1p and Boi2p stimulate vesicle fusion at the plasma membrane of yeast cells.
 J Cell Sci. 2017; 130(18):2996-3008

Publications 2016

Glomb O, Gronemeyer T. 
Septin organization and functions in budding yeast. Review. 
Front. Cell Dev. Biol. 2016; 4:123.

Gronemeyer T, Chollet J, Werner S, Glomb O, Bäuerle A, Johnsson N. 
A Split-Ubiquitin based strategy selecting for protein complex-interfering mutations.
G3 (Bethesda). 2016; 6(9):2809-15.

Gronemeyer T, Suarez W, Nuytemans H, Calaramo M, Wistuba A, Mey FS, Amoroso VB.
Two new Nepenthes species from the Philippines and an emended description of Nepenthes ramos. 
Plants. 2016; 5(2):23.

Renz C, Oeljeklaus S, Grinhagens S, Warscheid B, Johnsson N, Gronemeyer T.
Identification of cell cycle dependent interaction partners of the septins by quantitative mass spectrometry. 
PLoS One. 2016, 11(2):e0148340.

Brausemann A, Gerhardt S, Schott AK, Einsle O, Große-Berkenbusch A, Johnsson N, Gronemeyer T. 
Crystal structure of Cdc11, a septin subunit from Saccharomyces cerevisiae. 
J Struct Biol. 2016, 193, 157-161

Publications 2015

Dünkler A, Rösler R, Kestler HA, Moreno-Andrés D, Johnsson N.
SPLIFF: A Single-Cell Method to Map Protein-Protein Interactions in Time and Space.
Methods Mol Biol. 2015;1346:151-68

Ishitsuka Y, Azadfar N, Kobitski AY, Nienhaus K, Johnsson N, Nienhaus GU.
Evaluation of Genetically Encoded Chemical Tags as Orthogonal Fluorophore Labeling Tools for Single-Molecule FRET Applications.
J Phys Chem B. 2015, 119(22):6611-9

Kaplan C, Jing B, Winterflood CM, Bridges AA, Occhipinti P, Schmied J, Grinhagens S, Gronemeyer T, Tinnefeld P, Gladfelter A, Ries J, Ewers H. 
The absolute arrangement of subunits in cytoskeletal septin filaments in cells measured by fluorescence microscopy. 
Nano Lett. 2015, 15(6):3859-64.

Neller J, Dünkler A, Rösler R, Johnsson N
A protein complex containing Epo1p anchors the cortical endoplasmic reticulum to the yeast bud tip.
J Cell Biol. 2015, 208(1):71-87

Publications 2014

Barth H, Johnsson N.
Mitotic entry elucidated with bacterial toxin toolbox.
Cell Cycle. 2014, 13(14), 2159

Tian C, Wu Y, Johnsson N.
Stepwise and cooperative assembly of a cytokinetic core complex in yeast Saccharomyces cerevisiae.
J Cell Sci. 2014, 127(Pt 16):3614-24

Gronemeyer T, Coritico F, Wistuba A, Marwinski D,  Gieray T, Micheler M, Mey F, Amoroso V. 
Four new species of Nepenthes L. (Nepenthaceae) from the central mountains of Mindanao, Philippines.
Plants 2014, 3, 284-303

Johnsson N.
Analyzing protein-protein interactions in the post-interactomic era. Are we ready for the endgame?
Biochem Biophys Res Commun. 2014, 445(4):739-45

Publications 2013

Renz C, Johnsson N, Gronemeyer T. 
An efficient protocol for the purification and labeling of entire yeast septin rods from E.coli for quantitative in vitro experimentation.
BMC Biotechnol 2013; 13(1):60

Schneider C, Grois J, Renz C, Gronemeyer T, Johnsson N. 
Septin rings act as template for myosin higher-order structures and inhibit redundant polarity establishment.
J Cell Sci. 2013; 126(Pt 15):3390-400

Moreno D, Neller J, Kestler HA, Kraus J, Dünkler A, Johnsson N.
A fluorescent reporter for mapping cellular protein-protein interactions in time and space.
Mol Systems Biol 2013, 9: 647

Gronemeyer T, Wiese S, Ofman R, Bunse C, Pawlas M, Hayen H, Eisenacher M, Stephan C, Meyer HE, Waterham HR, Erdmann E, Wanders RJ, Warscheid B.
The proteome of human liver peroxisomes: Identification of five new peroxisomal constituents by a label-free quantitative proteomics survey.
PLoS-ONE 2013; 8(2): e57395 

Gronemeyer T, Wiese S, Grinhagens S, Schollenberger L, Satiagraha A, Huber LA, Warscheid B, Just W. 
Localization of Rab proteins to peroxisomes: a proteomics and immunofluorescence study.
FEBS Lett. 2013; 587(4):328-38

Publikationen 2012

Labedzka K, Tian C, Nussbaumer U, Timmermann S, Walther P, Müller J, Johnsson N.
Sho1p connects the plasma membrane with proteins of the cytokinesis network via multiple isomeric interaction states.
J Cell Sci. 2012; 125:4103-13

Dünkler A, Müller J, Johnsson N.
Detecting protein-protein interactions with the split-ubiquitin sensor.
Methods Mol Biol. 2012; 786:115-30.

Wiese S, Gronemeyer T, Brites P, Ofman R, Bunse C, Renz C, Meyer HE, Wanders RJA, Warscheid B.
Comparative profiling of the peroxisomal proteome of wildtype and Pex7 knockout mice by quantitative mass spectrometry
Int J Mass Spectrom 2012; 312: 30– 40 

Publications 2011

Hruby A, Zapatka M, Heucke S, Rieger L, Wu Y, Nussbaumer U, Timmermann S, Dünkler A, Johnsson N.
A constraint network of interactions: protein-protein interaction analysis of the yeast type II phosphatase Ptc1p and its adaptor protein Nbp2p.
J Cell Sci. 2011; 124(Pt 1):35-46.

Publications 2010

Schollenberger L, Gronemeyer T, Huber CM, Lay D, Wiese S, Meyer HE, Warscheid B, Saffrich R, Peränen J, Gorgas K, Just W.
RhoA Regulates Peroxisome Association to Microtubules and the Actin Cytoskeleton. 
PLoS ONE 2010; 5(11): e13886

Cailleteau L, Estrach S, Thyss R, Boyer L, Doye A, Domange B, Johnsson N, Rubinstein E, Boucheix C, Ebrahimian T, Silvestre JS, Lemichez E, Meneguzzi G, Mettouchi A.
Alpha-2-beta-1 integrin controls association of Rac with the membrane and triggers quiescence of endothelial cells.
J Cell Sci. 2010;123(Pt 14):2491-501

Publications 2008

Müller J, Johnsson N.
Split-Ubiquitin and the Split-Protein Sensors: Chessman for the Endgame.
Chembiochem. 2008 Aug 1;9(13):2029-2038. 

Older publications

Johnsson N, Johnsson K.
Chemical tools for biomolecular imaging.
ACS Chem Biol. 2007 Jan 23;2(1):31-8. 

Reichel C, Johnsson N.
The split-ubiquitin sensor: measuring interactions and conformational alterations of proteins in vivo.
Methods Enzymol. 2005;399:757-76.

Vivero-Pol L, George N, Krumm H, Johnsson K, Johnsson N.
Multicolor imaging of cell surface proteins.
J Am Chem Soc. 2005 Sep 21;127(37):12770-1. 

Wang X, Johnsson N.
Protein kinase CK2 phosphorylates Sec63p to stimulate the assembly of the endoplasmic reticulum protein translocation apparatus.
J Cell Sci. 2005 Feb 15;118(Pt 4):723-32.

Johnsson N, George N, Johnsson K.
Protein chemistry on the surface of living cells.
Chembiochem. 2005 Jan;6(1):47-52. 

George N, Pick H, Vogel H, Johnsson N, Johnsson K.
Specific labeling of cell surface proteins with chemically diverse compounds.
J Am Chem Soc. 2004 Jul 28;126(29):8896-7.

Tafelmeyer P, Johnsson N, Johnsson K.
Transforming a (beta/alpha)8--barrel enzyme into a split-protein sensor through directed evolution.
Chem Biol. 2004 May;11(5):681-9. 

Johnsson N, Johnsson K.
A fusion of disciplines: chemical approaches to exploit fusion proteins for functional genomics.
Chembiochem. 2003 Sep 5;4(9):803-10. 

Eckert JH, Johnsson N.
Pex10p links the ubiquitin conjugating enzyme Pex4p to the protein import machinery of the peroxisome.
J Cell Sci. 2003 Sep 1;116(Pt 17):3623-34.  

Kindermann M, George N, Johnsson N, Johnsson K.
Covalent and selective immobilization of fusion proteins.
J Am Chem Soc. 2003 Jul 2;125(26):7810-1. 

Johnsson N.
A split-ubiquitin-based assay detects the influence of mutations on the conformational stability of the p53 DNA binding domain in vivo.
FEBS Lett. 2002 Nov 6;531(2):259-64.

Wittke S, Dünnwald M, Albertsen M, Johnsson N.
Recognition of a subset of signal sequences by Ssh1p, a Sec61p-related protein in the membrane of endoplasmic reticulum of yeast Saccharomyces cerevisiae.
Mol Biol Cell. 2002 Jul;13(7):2223-32.

Dues G, Müller S, Johnsson N.
Detection of a conformational change in G gamma upon binding G beta in living cells.
FEBS Lett. 2001 Sep 7;505(1):75-80.

Raquet X, Eckert JH, Müller S, Johnsson N.
Detection of altered protein conformations in living cells.
J Mol Biol. 2001 Jan 26;305(4):927-38.

Laser H, Bongards C, Schüller J, Heck S, Johnsson N, Lehming N.
A new screen for protein interactions reveals that the Saccharomyces cerevisiae high mobility group proteins Nhp6A/B are involved in the regulation of the GAL1 promoter.
Proc Natl Acad Sci U S A. 2000 Dec 5;97(25):13732-7.

Wittke S, Dünnwald M, Johnsson N.
Sec62p, a component of the endoplasmic reticulum protein translocation machinery, contains multiple binding sites for the Sec-complex.
Mol Biol Cell. 2000 Nov;11(11):3859-71. 

Wittke S, Lewke N, Müller S, Johnsson N.
Probing the molecular environment of membrane proteins in vivo.
Mol Biol Cell. 1999 Aug;10(8):2519-30.

Dünnwald M, Varshavsky A, Johnsson N.
Detection of transient in vivo interactions between substrate and transporter during protein translocation into the endoplasmic reticulum.
Mol Biol Cell. 1999 Feb;10(2):329-44.

Stagljar I, Korostensky C, Johnsson N, te Heesen S.
A genetic system based on split-ubiquitin for the analysis of interactions between membrane proteins in vivo.
Proc Natl Acad Sci U S A. 1998 Apr 28;95(9):5187-92.

Lévy F, Johnsson N, Rümenapf T, Varshavsky A.
Using ubiquitin to follow the metabolic fate of a protein.
Proc Natl Acad Sci U S A. 1996 May 14;93(10):4907-12.

Johnsson N, Varshavsky A.
Split ubiquitin as a sensor of protein interactions in vivo.
Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10340-4. 

Johnsson N, Varshavsky A.
Ubiquitin-assisted dissection of protein transport across membranes.
EMBO J. 1994 Jun 1;13(11):2686-98