Protein structure determination by cryo-EM

Together with our collaborator, Nikolaus Grigorrieff at Janelia Farm (U.S.A.), we have established electron cryo microscopy as a method to study the structure of amyloid fibrils. At Ulm University we have now set up a JEOL JEM 1400 and a JEM 2100 electron microscope that is equipped with a DE12 direct electron detector.

Selected references:

Schmidt A, Annamalai K, Schmidt M, Grigorieff N, Fändrich M
Cryo-EM reveals the steric zipper structure of a light chain-derived amyloid fibril.
Proc. Natl. Acad. Sci. U.S.A. 2016, 113, 6200-6205

Sachse C, Grigorieff N, Fändrich M
Nanoscale flexibility parameters of Alzheimer amyloid fibrils determined using electron cryomicroscopy.
Angewandte Chemie Int. Edt. 2010, 49, 1321-1323

Schmidt M, Sachse C, Richer W, Xu C, Fändrich M, Grigorieff N
Comparison between Alzheimer's Ab(1-40) and Ab(1-42) amyloid fibrils reveals similar protofilament structures.
Proc. Natl. Acad. Sci. U.S.A. 2009, 106, 19813-19818

Meinhardt J, Sachse C, Hortschansky P, Grigorieff N, Fändrich M
Aβ(1-40) fibril polymorphism implies diverse interaction patterns in amyloid fibrils.
J. Mol. Biol. 2009, 386, 869-877


Dr. Matthias Schmidt
Tel: +49-(0)731/50 32752
Fax: +49-(0)731/50 32759
Helmholtzstraße 8/1
Room number 2.45