Electron microscopy

Together with our collaborator, Nikolaus Grigorrieff at Janelia Farm (U.S.A.), we have established electron cryo microscopy as a method to study the structure of amyloid fibrils. At Ulm University we have now set up a JEOL JEM 1400 and a JEM 2100 electron microscope that is equipped with a DE12 direct electron detector.

Selected references:

Sachse C, Fändrich M, Grigorieff N
Paired β-sheet structure of an Aβ(1-40) amyloid fibril revealed by electron microscopy.
Proc. Natl. Acad. Sci. U.S.A. 2008, 105, 7462-7466

Schmidt M, Sachse C, Richer W, Xu C, Fändrich M, Grigorieff N
Comparison between Alzheimer's Ab(1-40) and Ab(1-42) amyloid fibrils reveals similar protofilament structures.
Proc. Natl. Acad. Sci. U.S.A. 2009, 106, 19813-19818

Meinhardt J, Sachse C, Hortschansky P, Grigorieff N, Fändrich M
Aβ(1-40) fibril polymorphism implies diverse interaction patterns in amyloid fibrils.
J. Mol. Biol. 2009, 386, 869-877

Sachse C, Xu C, Wieligmann K, Diekmann S, Grigorieff N, Fändrich M
Quaternary structure of a mature Alzheimer's b-amyloid fibril.
J. Mol. Biol. 2006, 362, 347-354