J. Andrecka, B. Treutlein, M. Izquierdo Arcusa, A. Muschielok, R. Lewis, A. Cheung, P. Cramer, and J. Michaelis
Nano positioning system reveals the course of upstream and nontemplate DNA within the RNA polymerase II elongation complex
Using our recently developed Nano-Positioning System we map the position of dye molecules attached to seven different locations along the nontemplate strand in Pol II elongation complex. The quality of the data allows us to build a model for the pathway of the nontemplate and upstream DNA thus completing our picture of the Pol II elongation complex.
Nucleic Acids Research, 37 (2009) 5803-5809


J. Andrecka, R. Lewis, F. Brückner, E. Lehmann, P. Cramer, and J. Michaelis
Single-molecule tracking of mRNA exiting from RNA polymerase II
Single-molecule FRET is used to map the position of the nascent RNA in RNA polymerase II elongation complexes. In the paper we use a novel hybrid approach of single-molecule fluorescence data and high resolution structural models. In analogy to satellites in the global positioning system (GPS), single-dye molecules act as nanoscopic reporters at known locations. Single-pair FRET can than be used to accurately determine a desired position of a flexible domain in large protein-nucleic acid complexes.
PNAS, 105 (2008) 135-140