PhD thesis:

Magnetic tweezers and fluorescence microscope for force spectroscopy experiments

Applying force on individual or interacting biomolecules is one of the most sensitive methodologies to investigate their energetic, kinetic and mechanistic properties. Force spectroscopy yields quantitative information such as interaction potentials and forces, pathways and intermediate states of reactions and reaction rate constants.  Combined with single molecule fluorescence microscopy and simulations, dynamic pictures with atomar resolution of biomolecular mechanisms and life processes can be obtained.  

In this project, you will build on our experience in instrument development and design and set up a novel magnetic tweezers microscope, combined with fluorescence microscopy, for quantitative single molecule experiments with highest sensitivity and spatial and temporal resolution. We recently discovered new modalities for the highly sensitive application of force and torque in our group, that you will further explore. You will apply the new instrument to fascinating biophysical questions and combine experimental findings with theoretical modeling.
The project is ideally suited for physics students with a strong background in optics and high interest in biophysical questions.

If you are motivated and interested in working in a young, dynamic and interdisciplinary research group at the exciting frontier between Physics and Biology and at the forefront of technical possibilities, please send an email to: Christof Gebhardt