Biophysics Lab Course, Institute of Biophysics
Idea: In this experiment we identify and characterize proteins from short peptide sequences using computational databases.
Online algorithms like BLAST, FASTA etc. are utilized to identify a protein from two short peptide fragments.
The proteomics tools of the ExPASy portal are used to explore the biochemical characteristics of the target protein.
The 3D structure of the protein is downloaded from the PDB and viewed with molecular visualization programmes such as Chimera.
ClustalOmega and Consurf tools are used to analyse homology information and find evolutionary relations for the protein of interest.
Experiments: Gene expression | Bioinformatics | Protein labelling | Protein crystallization | Protein folding | Stopped-flow kinetics | Single-molecule fluorescence | Fluorescence lifetime | Ligand binding | Fluorescence correlation spectroscopy (FCS) | Lipid monolayers | Ion channels | Live cell imaging | Superresolution microscopy | Optical tweezers | AFM
