Biophysics Lab Course, Institute of Biophysics
Idea: We learn how AFM experiments can be used to investigate the local mechanical properties of cells.
Topography and local stiffness of fixed mouse fibroblasts (cell line NIH/3T3 ATCC) is investigated with an atomic force microscope in force-volume mode.
Young’s modulus of the cells is determined and compared to cells, which were previously treated with cytochalasin D, a fungal toxin that inhibits actin polymerization and elongation.
Experiments: Gene expression | Bioinformatics | Protein labelling | Protein crystallization | Protein folding | Stopped-flow kinetics | Single-molecule fluorescence | Fluorescence lifetime | Ligand binding | Fluorescence correlation spectroscopy (FCS) | Lipid monolayers | Ion channels | Live cell imaging | Superresolution microscopy | Optical tweezers | AFM
